RNA Details
Disease Name
pulmonary fibrosis
Tissue
lung
RNA Symbol
Acta2
RNA ID
RNA Type
mRNA
Alteration Pattern
dna methylation
Species
rattus norvegicus
Detection Methods
qRT-PCR; DNA methyltransferase assay; etc.
Target
NA
Pathway
NA
PubMed ID
Title
Epigenetic regulation of myofibroblast differentiation by DNA methylation
Year
2010
Function
"Inhibition of DNA methyltransferase activity or knock down of DNA methyltransferase using specific small interfering RNA caused significant induction of alpha-SMA in fibroblasts. In contrast, induced overexpression of DNA methyltransferase suppressed alpha-SMA gene expression. Transforming growth factor beta induced myofibroblast differentiation was enhanced or suppressed by knockdown or overexpression of DNA methyltransferase, respectively. Finally, in vitro DNA methylation of the alpha-SMA promoter suppressed its activity. These findings suggest that DNA methylation mediated by DNA methyltransferase is an important mechanism regulating the alpha-SMA gene expression during myofibroblast differentiation."